Molecular Characterization of Rice Genotypes Using Molecular Markers

Rice, (Oryza sativa L. 2n=24) belongs to the family Gramineae and subfamily Oryzoideae. Oryza has two cultivated species: glaberrima. Breeding for good-quality traits requires selection of parents with a wider genetic diversity. Molecular markers are used in molecular biology biotechnology identify sequence DNA. have played an increasing role rice breeding cultivar improvement, screening, selection, germplasm collections. The present investigation is undertaken study diversity among thirty genotypes using ISSR marker.
 Out 40 primers, 9 amplified showed polymorphism, viz., 807, 808, 809, 811, 816, 823, 826, 827 829. A total 76 loci were generated by amplification polymorphic out which 66 average 86.84 percent polymorphism. Among 807 produced maximum number 11 loci. dendrogram constructed NTSYSpc 2.02i software grouped all 30 into major clusters (clusters B). Cluster Kalbhat alone one sub-cluster at 69.2% similarity Karjat 2, Jaya, Sairam, Saubhagya Dhan, RPBIO 226, 6, Ratnagiri 1, DRR Dhan 44, 45, 46, MTU 10010, 7, Swarna Shreya, Sugandha, Madhumati. In B 3 forms sub- cluster 70 % 1001, PKVHMT, JGL 1798, Indrayani, Phule Samruddhi, Maval, Sonsal, Pavana, 41, kundalika, Bhogawati, Ambemohar, Radha.